Jie Xiao, PhD
Jie grew up in Chongqing, a city upstream of Yangtze river in China. She then went to Nanjing, a city downstream of Yangtze river to receive her undergraduate education in Biochemistry at Nanjing University. In 1997, Jie came to the United States and enrolled in the Graduate Program in Biochemistry and Cell Biology at Rice University. She worked with Prof. Scott F. Singleton on the mechanism of RecA mediated strand exchange reaction. Later in her graduate years she found herself deeply fascinated by single molecule biophysics and joined Prof. Sunney Xie’s group as a postdoctoral fellow at Harvard University in 2002. She spent four good years learning single molecule fluorescence microscopy and physical chemistry, while contributing to the group with her biological background and perspectives. In 2006, she decided to escape the cold winter of Boston and found her home in the Department of Biophysics and Biophysical Chemistry at Johns Hopkins University. She is continuing the line of research where she takes single molecule assays into living cells to probe many different aspects of cellular dynamics. In addition to working in the lab, Jie loves the beautiful weather of Maryland and spicy food.
My laboratory focuses on developing novel single-molecule imaging tools in live cells to probe various dynamic aspects of cellular processes. In the past we have developed novel single-molecule gene expression reporting systems and chromosomal DNA conformation markers to probe the dynamic of gene regulation and transcription in live bacterial cells. We also pioneered the use of superresolution imaging to probe the structure, function and dynamics of the bacterial cell division machinery. Our work is at the frontier of imaging dynamic cellular processes, and has enabled new quantitative understandings of the dynamics of gene regulation and cell division. Recently we expanded our horizons by collaborating with experts of different fields to map the spatial organization of a single human cell’s genome and epigenetic markers, and to develop new single-molecule based technologies for sensitive early detection of cancer markers in blood samples.
Jie Xiao is a molecular biologist and biophysicist who focuses on dissecting mechanisms in gene regulation and cytokinesis in the model organism E. coli.